Datasheet: TheOne™ RNA Reagent

Description：
TheOne Reagent is a single reagent developed for the isolation of total RNA from animal and plant tissue, cells and bacteria culture. This reagent can also simultaneously extracted DNA and proteins for southern blot, PCR and western blot analysis. The entire procedure for total RNA isolation is an improvement single-step method developed by Chomczynski and Sacchi. After homogenization of sample and chloroform extraction, three phases are formed ( aqueous phase, interphase and organic phase ). RNA can be precipitated by isopropanol from aqueous phase, DNA can be recovered by ethanol precipitation from interphase, and proteins are precipitated with isopropanol from organic phase. The reagent also includes a bottle of De-PSG Solution, which will eliminate polysaccharides and proteoglycans contamination, the RNA will be dissolve easier and purer for RT-PCR and northern blot application.